Publications

Publications (full papers and review articles)

ESR names in bold. In order of most recent publication date.

  1. Cao Y., Wang, Y., Sprangers, S., Picavet, D.I., Glogauer, M., McCulloch, C.A., Everts, V. (2017) Deletion of adseverin in osteoclasts affects cell structure but not bone metabolism. Calcif. Tissue Int. Published online 07 April 2017.
    DOI: 10.1007/s00223-017-0271-6
  2. Montano Almendras, C. P., Thudium, C. S., Löfvall, H., Moscatelli, I., Schambach, A., Henriksen, K., & Richter, J. (2017), Forced expression of human M-CSF in CD34+ cells promotes monocyte differentiation in vitro and in vivo but blunts osteoclastogenesis in vitro. Eur. J. Haematol. Accepted Author Manuscript.
    DOI:10.1111/ejh.12867
  3. Sprangers, S., Schoenmaker, T., Cao, Y., Everts, V., & de Vries, T.J. (2017) Integrin αMβ2 is differently expressed by subsets of human osteoclast precursors and mediates adhesion of classical monocytes to bone. Exp. Cell Res. 350, 161-168. DOI: 10.1016/j.yexcr.2016.11.018
  4. Sprangers, S., Behrendt, N., Engelholm, L., Cao, Y., & Everts, V. (2016) Phagocytosis of collagen fibrils by fibroblasts in vivo is independent of the uPARAP/Endo180 receptor. J. Cell. Biochem. 2016 Dec 6. [Epub ahead of print]
    DOI: 10.1002/jcb.25821
  5. Di Ceglie, I., van den Akker, G.G., Ascone, G., Ten Harkel B., Häcker, H., van de Loo, F.A., Koenders, M.I., van der Kraan, P.M., de Vries, T.J., Vogl, T., Roth, J., & van Lent, P.L. (2016) Genetic modification of ER-Hoxb8 osteoclast precursors using CRISPR/Cas9 as a novel way to allow studies on osteoclast biology. J. Leukoc. Biol. 2016 Dec 5. [Epub ahead of print]
    DOI: 10.1189/jlb.1AB0416-180RR
  6. Sztacho, M., Segeletz, S., Sanchez-Fernandez, M.A., Czupalla, C., Niehage, C., Hoflack, B. (2016) BAR Proteins PSTPIP1/2 regulate podosome dynamics and the resorption activity of osteoclasts. PLoS One. 2016;11(10):e0164829.
    DOI: 10.1371/journal.pone.0164829
  7. Cao, Y., Jansen, I.D.C., Sprangers, S., Stap, J., Leenen, P.J.M., Everts, V., & de Vries, T.J. (2016) IL-1β differently stimulates proliferation and multiucleation of distinct mouse bone marrow osteoclast precursor subsets. J. Leukoc. Biol. 100, 513-523.
    DOI: 10.1189/jlb.1A1215-543R
  8. Thudium, C.S., Moscatelli, I., Löfvall, H., Kertész, Z., Montano, C., Bjurström, C.F., Karsdal, M.A., Schulz, A., Richter, J., & Henriksen, K. (2016) Regulation and function of lentiviral vector-mediated TCIRG1 expression in osteoclasts from patients with infantile malignant osteopetrosis: implications for gene therapy. Calcif. Tissue Int. 99, 638-648.
    DOI: 10.1007/s00223-016-0187-6
  9. Segeletz, S., & Hoflack, B. (2016) Proteomic approaches to study osteoclast biology. Proteomics 16, 2545-2556.
    DOI: 10.1002/pmic.201500519
  10. Sprangers, S., De Vries, T.J., & Everts, V. (2016) Monocyte heterogeneity: consequences for monocyte-derived immune cells. J. Immunol. Res. 3, 1-10.
    DOI: 10.1155/2016/1475435
  11. Sprangers, S., Schoenmaker, T., Cao, Y., Everts, V., & de Vries, T.J. (2015) Different blood-borne human osteoclast precursors respond in distinct ways to IL-17A. J. Cell. Physiol. 231, 1249-1260.
    DOI: 10.1002/jcp.25220

Conference presentations

Euroclast held a consortium meeting in Rome, prior to attending the conference of the European Calcified Tissue Society 2016.

All ESRs presented at the ECTS consortia summit and again during the conference.

  1. Vikte Lionikaite, Karin Gustafsson, Anna Westerlund, Antti Koskela, Juha Tukkanen, H. Herschel Conaway, Claes Ohlsson, Petra Henning & Ulf Lerner. Effects of Clinically Relevant Doses of Vitamin A on Bone in Mice. Bone Abstracts (2016) 5 P280.
    DOI:10.1530/boneabs.5.P280
  2. Henrik Löfvall, Christian Schneider Thudium, Ilana Moscatelli, Carmen Montano, Ansgar Schulz, Johan Richter & Kim Henriksen. Regulation and function of lentiviral-mediated TCIRG1 expression in osteoclasts from infantile malignant osteopetrosis patients. Bone Abstracts (2016) 5 P192.
    DOI:10.1530/boneabs.5.P192
  3. Giuliana Ascone, Irene Di Ceglie, Wouter de Munter, Birgitte Walgreen, Annet Sloetjes & Peter van Lent. Apolipoprotein E aggravates inflammation and bone destruction in murine antigen - induced arthritis. Bone Abstracts (2016) 5 P9.
    DOI:10.1530/boneabs.5.P9
  4. Emma McDermott, David Sunderhauf, Kevin Mackenzie, Debbie Wilkinson & Miep Helfrich. Identification and characterisation of vesicles in resorbing osteoclasts using electron tomography. Bone Abstracts 5 P190.
    www.bone-abstracts.org/ba/0005/ba0005p190.htm
    DOI:10.1530/boneabs.5.P190
  5. Anh Tran, Fraser Coxon, Emma McDermott, Ian Ganley, Paul Odgren, Jennifer Martinez, Douglas Gree &, Miep Helfrich. The role of LC3 and autophagy in bone resorption by osteoclasts. Bone Abstracts (2016) 5 P195.
    DOI:10.1530/boneabs.5.P195
  6. David Massa Lopez, Markus Damme & Paul Saftig. Characterising the role of the lysosomal membrane proteins MFSD1 and TMEM106b in osteoclasts. Bone Abstracts (2016) 5 P185.
    DOI:10.1530/boneabs.5.P185
  7. Sara Sprangers, Ton Schoenmaker, Yixuan Cao, Vincent Everts & Teun J. de Vries. Involvement of integrin Beta 2/CD18 in attachment of monocytes to bone. Bone Abstracts (2016) 5 P184.
    DOI:10.1530/boneabs.5.P184
  8. Yixuan Cao, Ineke Jansen, Sara Sprangers, Jan Stap, Vincent Everts & Teun de Vries. Life span differs between osteoclasts derived from different bone marrow precursors : a time-lapse microscopy study. Bone Abstracts (2016) 5 P183.
    DOI:10.1530/boneabs.5.P183
  9. Sandra Segeletz, & Bernard Hoflack. The Arf and Rho GTPase-activating protein (GAP) ARAP1 regulates osteoclast function. Bone Abstracts 5 P188.
    DOI:10.1530/boneabs.5.P188
  10. Laia Mira Pascual, Pernilla Lång & Göran Andersson RAW264.7 subclones – an in vitro model for osteoclast heterogeneity? Bone Abstracts 5 P200.
    DOI:10.1530/boneabs.5.P200
  11. Christina Patlaka, Laia Mira-Pascual, Staffan Paulie, Pernilla Lång & Göran Andersson Development of a novel sandwich ELISA to quantify human Tartrate Resistant Acid Phosphatase (TRAP) isoforms 5a and 5b protein in one and the same sample. Bone Abstracts (2016) 5 P198.
    DOI:10.1530/boneabs.5.P198

A number of Euroclast ESRs presented their projects at the conference of the European Calcified Tissue Society in Rotterdam in April 2015. During this meeting Euroclast also presented a consortium poster highlighting all the research projects and training activities undertaken by its ESRs

  1. Laia Mira Pascual, Christina Patlaka, Pernilla Lång & Göran Andersson.  Tartrate resistant acid phosphatase 5a – a coupling factor between osteoclast and osteoclast with potential growth factor activity? Article number: 673 (2015) P204.
    DOI:1.1038/bonekey.2015.40
  2. Sara Sprangers, Ton Schoemaker, Yixuan Cao, Vincent Everts & Teun de Vries. Different human osteoclast precursors respond in distinct ways to IL-17A. IBMS BoneKEy 13. Article number: 673 (2015) P211.
    DOI:1.1038/bonekey.2015.40
  3. Yixuan Cao, Ineke Jansen, Sara Sprangers, Vincent Everts & Teun de Vries. IL1-β differently affects osteoclastogenesis of distinct subsets of osteoclast precursors. IBMS BoneKEy 13. Article number: 673 (2015) P212.
    DOI:1.1038/bonekey.2015.40

Presentations at other international meetings

  1. Giuliana Ascone Apolipoprotein E aggravates inflammation and cartilage destruction in murine antigen - induced arthritis. European Workshop for Rheumatology Research 2017. Poster presentation.
  2. Ascone, G., Di Ceglie, I., de Munter, W., Walgreen, B., Sloetjes, A., van Lent, P., & van der Kraan, P. Apolipoprotein E aggravates inflammation and bone destruction in murine antigen - induced arthritis. European League Against Rheumatism, London, 2016. Oral presentation. Ann. Rheum. Dis. 2016; 75 (Suppl.2):136
  3. Massa López, D., Lüllmann-Rauch, R., Saftig, P. & Damme M.(2015) Characterization of MFSD1-a new lysosomal protein- and its physiological role in the mouse. European Study Group on Lysosomal Diseases (ESGLD). https://www.esgld.org/past-esgld-workshops/ P57

Presentations at national meetings

  1. Emma McDermott, Debbie Wilkinson, Kevin Mackenzie, Justin Rochford, & Miep Helfrich (2016) Visualising osteoclast ruffled border formation using electron tomography. Scottish Microscopy Group Meeting Edinburgh.
  2. Sara Sprangers, Yolande Ramos, Ton Schoenmaker, Yixuan Cao, Vincent Everts, Ingrid Meulenbelt, &cTeun J. de Vries. Patients with OA due to a gain-of-function OPG mutation have affected osteoclastogenesis. Nederlandse Vereniging voor Calcium- en Botstofwisseling 2016. Oral presentation (SS).
  3. Yixuan Cao, Ineke Jansen, Sara Sprangers, Teun J. de Vries, & Vincent Everts (2016) TNF-α prevents osteoclast formation by mouse monocytes due to interfering with RANKL- RANK interaction. Nederlandse Vereniging voor Calcium- en Botstofwisseling 2016. Oral presentation (YC).
  4. David Sünderhauf, Emma McDermott, Debbie Wilkinson, Kevin Mackenzie, & Miep Helfrich. Identification and characterisation of extracellular vesicles and uptake events in resorbing osteoclasts using electron tomography. 2016. Poster pitching (DS) and poster presentation. http://scottishmicroscopygroup.org.uk/symposia/2016-symposium%20Poster%20presentation P50.
  5. Anh Tran, Fraser Coxon, Emma McDermott, Angela Douglass, Diana Miranda de Stegmann, Ian Ganley, Paul Odgren, Wim van Hul, & Miep Helfrich (2016) The role of LC3 and autophagy in bone resorption by osteoclasts. The 2nd Autophagy UK meeting. Edinburgh. http://autophagy.uk/meeting2016/ Oral presentation (AT).
  6. Sara Sprangers, Ton Schoenmaker, Yixuan Cao, Vincent Everts, & Teun J. de Vries. Interaction between different osteoclast precursors and bone. Nederlandse Vereniging voor Calcium- en Botstofwisseling 2015. Oral presentation (SS). Awarded with the presentation price.
  7. Yixuan Cao, Ineke Jansen, Sara Sprangers, Jan Stap, Vincent Everts & Teun J. de Vries. A diverse stimulatory role of IL-1β on different murine osteoclast precursor subsets: a microscopy study. Nederlandse Vereniging voor Calcium- en Botstofwisseling 2015. Oral presentation (YC).
  8. Sara Sprangers, Ton Schoenmaker, Yixuan Cao, Vincent Everts, & Teun J. de Vries Different human osteoclast precursors respond in distinct ways to IL-17A. Nederlandse Vereniging voor Calcium- en Botstofwisseling 2014. Oral presentation (SS).
  9. Yixuan Cao, Ineke Jansen, Sara Sprangers, Vincent Everts, & Teun J. de Vries. IL-1beta differently affects osteoclastogenesis of distinct osteoclast precursor subsets. Nederlandse Vereniging voor Calcium-en Botstofwisseling 2014. Oral presentation (YC).

 

Several Euroclast ESRs attended the ECTS PhD training course in Sienna, Italy, September 2015 and presented their work.

Emma McDermott, Kevin Mackenzie, Fraser Coxon, & Miep Helfrich. Characterisation of osteoclast vesicular compartment and ruffled border ultrastructure using advanced imaging. Poster presentation.

Vikte Lionikaite. The role of retinoids in bone remodelling. Oral Presentation.

David Massa Lopez, Emma McDermott, Paul Saftig, & Damme. CD63 is implicated in differentiation of osteoclasts during stimulation with MCSF and RANKL. Poster presentation.

Sandra Segeletz & Bernard Hoflack.  Coupling post-Golgi transport with membrane dynamics in osteoclasts.  Oral presentation.

Sara Sprangers, Ton Schoenmaker, Yixuan Cao, Vincent Everts, & Teun J. de Vries. Different human osteoclast precursors respond in distinct ways to IL-17A. Poster presentation.

Yixuan Cao, Ineke Jansen, Sara Sprangers, Vincent Everts, & Teun de Vries. IL1-β differently affects osteoclastogenesis of distinct subsets of osteoclast precursors.  Poster presentation.

Anh Tran. Mechanisms/Molecules Essential in Ruffled Border Formation:  Focus on Autophagy.  Oral presentation.

Public outreach

See also the webpages of Euroclast for more information about public outreach.

Emma McDermott. Winner of 2nd prize in the Imaging Competition of the Scottish Microscopy Group 2016. http://scottishmicroscopygroup.org.uk/smg-image-competition.

David Massa Lopez, Markus Damme and Paul Saftig. European Researchers Night, 30th September 2016,Leibnizstraße, Christian-Albrechts-Universität zu Kiel. Presentation of “Euroclast” poster.

Yixuan Cao, Teun de Vries, Ineke Jansen. Vrije University Amsterdam anniversary event “Kowledge in movement”. Organization of workshop “Long live bone”, 16/04/2016, Amsterdam. http://www.euroclast.eu/documents/Euroclast_Newsletter_VU_openday.pdf

Emma Mc Dermott, Anh Tran, Miep Helfrich and Fraser Coxon. European Researchers Night, 25th September 2015, Union Square Shopping Centre, Aberdeen. Stand and “science busking” to promote bone health and the work done by the Euroclast consortium. http://www.euroclast.eu/about/outreach/

Emma Mc Dermott. Euroclast, understanding the Osteoclast. Paper in AU magazine. http://ausm.org.uk/wp content/uploads/2014/02/Issue11.pdf